Invitrogen™ TOP2B Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA527750

Catalog No. PIPA527750

Description

Recommended positive controls: Jurkat, K562, THP-1. Predicted reactivity: Mouse (89%), Rat (86%), Pig (88%), Rhesus Monkey (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: TOP2B
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TOP2B
• Gene Alias: antigen MLAA-44; D230016L12Rik; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; Top-2; Top2b; top2beta; TOPIIB; topo II beta; topoisomerase (DNA) II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21974, 361100, 7155
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 0.48 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: Q02880, Q64511
• Gene Symbols: TOP2B
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Topoisomerase II beta. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG