Invitrogen™ TOP3A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA575799

Catalog No. PIPA575799

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIalpha exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIalpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo IIIbeta exists as three isoforms,beta-1,beta -2 and beta-3, also produced by alternative splicing. Topo IIIbeta-1 is expressed in testis, heart, and skeletal muscle, whereas Topo IIIbeta-2 is expressed in thymus, kidney and pancreas.

Specifications

• Antigen: TOP3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Top3a
• Gene Alias: DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; Top IIIa; TOP3; TOP3A; topo III-alpha; topoisomerase (DNA) 3 alpha; topoisomerase (DNA) III alpha; topoisomerase 3 alpha; ZGRF7; zinc finger, GRF-type containing 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7156
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q13472
• Gene Symbols: Top3a
• Immunogen: Synthetic peptide corresponding to residues in Human TOP3A.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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