Invitrogen™ TOP3A Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5101160

Catalog No. PIPA5101160

Description

Antibody detects endogenous levels of total TOP3A.

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIalpha exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIalpha, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo IIIbeta exists as three isoforms,beta-1,beta -2 and beta-3, also produced by alternative splicing. Topo IIIbeta-1 is expressed in testis, heart, and skeletal muscle, whereas Topo IIIbeta-2 is expressed in thymus, kidney and pancreas.

Specifications

• Antigen: TOP3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Top3a
• Gene Alias: DNA topoisomerase 3-alpha; DNA topoisomerase III alpha; Top IIIa; TOP3; TOP3A; topo III-alpha; topoisomerase (DNA) 3 alpha; topoisomerase (DNA) III alpha; topoisomerase 3 alpha; ZGRF7; zinc finger, GRF-type containing 7
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 21975, 7156
• Content And Storage: -20°C
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O70157, Q13472
• Gene Symbols: Top3a
• Immunogen: A synthesized peptide derived from human TOP3A(Accession Q13472), corresponding to amino acid residues D953-R1001.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

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