Invitrogen™ Human TRAIL R4/ TNFRSF10D ELISA Kit

ELISA

Catalog Number	Mfr. No.	Quantity
EHTNFRSF10D	Invitrogen™ EHTNFRSF10D	96 Tests
50-274-1190	Invitrogen™ EHTNFRSF10DX5	5 x 96 Tests
50-255-0904	Invitrogen™ EHTNFRSF10DX10	10 x 96 Tests

Description

The Human TRAIL R4 TNFRSF10D (TRAIL R4) ELISA quantitates Hu TRAIL R4 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TRAIL R4. Principle of the method The Human TRAIL R4 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

TRAIL (also known as Apo-2L) is a member of the tumor necrosis factor (TNF) ligand family that rapidly induces apoptosis in a variety of transformed cell lines. A fourth member of the emerging TRAIL receptor family, TRAIL-R4, has signaling capacity similar to that of TRAIL-R1 and TRAIL-R2 with respect to NF-kappaB activation, but differs in its inability to induce apoptosis. Like TRAIL-R1 and TRAIL-R2, this receptor shows widespread tissue expression. The human TRAIL-R4 gene has been mapped to chromosome 8p22-21, clustered with three other TRAIL receptors.

Specifications

• Accession Number: Q9UBN6
• Assay Sensitivity: 10 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CD264,DCR2,TRAIL-R4,TRAILR4,TRUNDD
• Intraassay CV: <10
• Label or Dye: HRP
• Research Discipline: Apoptosis
• Storage Requirements: 2°C to 8°C
• Target: TRAIL-R4 (CD264)
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 5.487 to 4000 pg/mL
• Calibration Range: 10 pg/mL to 4000 pg/mL
• Conjugate: HRP
• Protein Family: Other Proteins
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 8793
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Synonym: UNQ251/PRO288, CD264, DCR2, TRAIL-R4, TRAILR4, TRUNDD
• Test Time: 1 hrs 20 mins