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Invitrogen™ Transferrin From Human Serum, Alexa Fluor™ Conjugate
Description
Fluorescent transferrin for receptor-mediated endocytosis and recycling
Fluorescent transferrin conjugates are widely used to study transferrin receptor-mediated endocytosis, endosomal trafficking, and recycling using live-cell imaging or fixed-cell endpoint analysis. Transferrin binds transferrin receptor 1 (TfR1) at the cell surface and is internalized through clathrin-mediated endocytosis. Following endosomal acidification and iron release, apo-transferrin remains bound to TfR1 and the complex recycles to the plasma membrane, where apo-transferrin dissociates.
Why use fluorescent transferrin for endocytosis studies?
- Track receptor-mediated endocytosis: Follow transferrin binding, TfR-mediated internalization, and intracellular trafficking.
- Monitor endocytic recycling: Use fluorescent transferrin as a well-established marker of the endosomal recycling pathway.
- Study trafficking kinetics: Follow uptake and recycling using live-cell imaging, pulse-chase experiments, or fixed-cell endpoint analysis.
- Assess endosomal trafficking: Investigate trafficking events associated with endosomal acidification, cargo sorting, and membrane transport.
- Compare trafficking pathways: Combine fluorescent transferrin with fluorescent LDL or other cargo markers to compare recycling and lysosomally directed pathways.
- Enable multiplex fluorescence imaging: Choose from fluorescein and Alexa Fluor™ 488, 546, 555, 568, 594, 633, and 647 conjugates for multicolor imaging workflows.
Fluorescent transferrin enables a direct fluorescence readout of a well-characterized endocytic pathway, making it a versatile probe for studying receptor internalization, endosomal trafficking, and recycling.
Choose a fluorescent transferrin conjugate for your imaging workflow
Select from fluorescein and Alexa Fluor™ 488, 546, 555, 568, 594, 633, and 647 transferrin conjugates, with fluorescence spanning green to far-red wavelengths.
Multiple fluorophore options
- Match your imaging system: Select a conjugate compatible with available excitation sources, emission filters, and detectors.
- Support multicolor imaging: Choose spectrally distinct labels for use with antibodies, organelle markers, and other fluorescent probes.
- Optimize experimental design: Select the excitation and emission profile that best fits your available fluorescence channels.
- Use one biological probe across workflows: Study transferrin receptor trafficking with fluorophore options suitable for a range of fluorescence microscopy applications.
Applications of fluorescent transferrin
Fluorescent transferrin is a well-established probe for studying transferrin receptor-mediated endocytosis and intracellular trafficking.
Use fluorescent transferrin to:
- Visualize receptor-mediated internalization and recycling using live-cell imaging or fixed-cell endpoint analysis
- Track endosomal trafficking using fluorescence and confocal microscopy
- Study trafficking events associated with endosomal acidification and changes in endosomal function
- Investigate transferrin receptor dynamics, including appropriately designed FRET-based studies
- Measure transferrin receptor binding in mammalian and parasite model systems
- Compare recycling and lysosomal trafficking using complementary fluorescent cargo markers
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Store in freezer (-5°C to -30°C) and protect from light. |
| Detection Method | Fluorescence |
| Product Type | Transferrin |
| Dye Type | Alexa Fluor Dyes |
| Form | Solid |
| pH | 7.2 |
| Product Line | Alexa Fluor |
| Quantity | 5 mg |
| Shipping Condition | Room Temperature |
| Excitation/Emission | 495/519 nm |
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For Research Use Only. Not for use in diagnostic procedures.
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