Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Education Supplies
  4. Biology Classroom Products
  5. Microbiology Classroom Kits
  6. S68653

Edvotek™ Transformation of E. coli with Green and Blue Fluorescent Protein Plasmids Classroom Kit
SDPSCI_ED

Catalog No. S68653
Click to view available options
Class Size:
10 Lab Groups
Transformation experiments contain antibiotics which are used for the selection of transformed bacteria. Students who have allergies to antibiotics such as penicilllin, ampicillin, kanamycin or tetracycine should not participate in this experiment. All components intended for educational research only. They are not to be used for diagnostic or drug purposes, nor administered to or consumed by humans or animals.
Transformation experiments contain antibiotics which are used for the selection of transformed bacteria. Students who have allergies to antibiotics such as penicilllin, ampicillin, kanamycin or tetracycine should not participate in this experiment. All components intended for educational research only. They are not to be used for diagnostic or drug purposes, nor administered to or consumed by humans or animals.

Description

Includes

instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ agar, Luria broth, petri dishes, sterile pipets and loops

Requires

  • Automatic micropipet (5-50μL) and tips
  • 2 water baths (37°C and 42°C)
  • Thermometer
  • Incubation oven (34°C and 37°C)
  • Pipet pumps or bulbs
  • Ice
  • Marking pens
  • Bunsen burner, hot plate or microwave oven
  • Hot gloves
  • Long-ave UV light (EDVOTEK No. 969 recommended)

Helps students develop an understanding of bacterial transformation by plasmid DNA

  • Enables students to observe the acquired phenotypic trait of green or blue fluorescent protein exhibited by transformed bacterial cells
  • Transformants acquire green (GFP) or blue (BFP) fluorescent protein genes contained in two different plasmids; the GFP gene was mutated to produce the blue protein
  • Transformed cells are selected based on antibiotic resistance
  • Colonies which express GFP or BFP protein are visibly green and pale blue and will fluoresce when exposed to long-wave UV light

Order Info

Class size: 10 experiments. Shipped ambient. Supercoiled pFluoroGreen™, Supercoiled pFluoroBlue™, ampicillin, and IPTG require -20°C storage. Note:Transformation cell slant should not be frozen.

Specifications

Product Type Transformation of E. Coli Kit
Grade 9 to Undergraduate
For Use With (Application) Helps students develop an understanding of bacterial transformation by plasmid dna
Includes Instructions, lyphocells, plasmid dna, iptg, ampicillin, transformation solution, readypour™ agar, luria broth, petri dishes, sterile pipets and loops
Requires Waterbath , 37°C incubation oven, microwave or hot plate, long wave UV lamp
Class Size 10 Lab Groups
Product Content Correction

Your input is important to us. Please complete this form to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.