Edvotek™ Transformation of E. coli with Green and Blue Fluorescent Protein Plasmids Classroom Kit
Helps students develop an understanding of bacterial transformation by plasmid DNA
Manufacturer: Edvotek™ 222
- Enables students to observe the acquired phenotypic trait of green or blue fluorescent protein exhibited by transformed bacterial cells
- Transformants acquire green (GFP) or blue (BFP) fluorescent protein genes contained in two different plasmids; the GFP gene was mutated to produce the blue protein
- Transformed cells are selected based on antibiotic resistance
- Colonies which express GFP or BFP protein are visibly green and pale blue and will fluoresce when exposed to long-wave UV light
Class size: 10 experiments. Shipped ambient. Supercoiled pFluoroGreen™, Supercoiled pFluoroBlue™, ampicillin, and IPTG require -20°C storage. Note:Transformation cell slant should not be frozen.
|10 lab groups|
|Instructions, lyphocells, plasmid dna, iptg, ampicillin, transformation solution, readypour™ agar, luria broth, petri dishes, sterile pipets and loops|
|Waterbath , 37°C incubation oven, microwave or hot plate, long wave UV lamp|
|Helps students develop an understanding of bacterial transformation by plasmid dna|
|Transformation Of E. Coli Kit|
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