Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247), Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24443500	Novus Biologicals NBP2444350.02MG	0.02 mg
NBP24443501	Novus Biologicals NBP2444350.1MG	0.1 mg
NBP24443502	Novus Biologicals NBP2444350.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Transgelin/TAGLN/SM22 alpha Monoclonal specifically detects Transgelin/TAGLN/SM22 alpha in Human, Mouse, Porcine, Bovine, Rabbit samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Transgelin/TAGLN/SM22 alpha
• Concentration: 0.2mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human, Mouse, Pig, Primate, Rabbit
• Gene Accession No.: Q01995
• Gene ID (Entrez): 6876
• Immunogen: Recombinant full-length human transgelin (TAGLN) protein.
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 22 kDa

• Clone: TAGLN/247
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: DKFZp686P11128, Protein WS3-10, SM2222 kDa actin-binding protein, SMCCDKFZp686B01212, Smooth muscle protein 22-alpha, TAGLN1, transgelin, transgelin variant 2, WS3-10SM22-alpha
• Gene Symbols: TAGLN
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

For Research Use Only