Invitrogen™ TRIM28 Recombinant Rabbit Monoclonal Antibody (BL-248-2G6)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA544223	Invitrogen™ MA544223	100 μL
PIMA544224	Invitrogen™ MA544224	20 μL

Description

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

The KRAB (Kruppel-associated box) domain is about 45 amino acids in length and is a transcriptional repression domain found in numerous transcription factors. There have been identified over 220 KRAB-zinc finger protein (KRAB-ZFP) genes in the human genome. These proteins functionally repress transcription via specific interactions with KAP-1 (KRAB-associated protein 1). KAP-1 is an 835 amino acid polypeptide that contains a RING finger, B boxes, and a PHD finger. KAP-1 has been shown to form complexes with KRAB-domain transcription factors and increase the efficiency with which they mediate repression. KAP-1 has also been shown to directly interact with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1). KAP-1 directly targets KRAZ1 to the foci of centromeric heterochromatin containing HP1alpha, thus helping to regulate transcriptional repression. Studies have shown that KAP-1 mutants with the ability to bind KRAB but unable to bind HP1 leads to random distribution of KRAZ1 and strong transcriptional activation.

Specifications

• Antigen: TRIM28
• Concentration: 40 μg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: BBS with 0.1% BSA and 0.09% sodium azide; pH 7.8-8.6
• Gene Accession No.: Q13263
• Gene ID (Entrez): 10155
• Immunogen: Synthetic peptide representing a portion of human KRAB-Associated Protein 1 surrounding Serine 824.
• Product Type: Antibody

• Clone: BL-248-2G6
• Content And Storage: 4°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Human
• Gene: TRIM28
• Gene Alias: AA408787; E3 SUMO-protein ligase TRIM28; KAP1; KAP-1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-A-interacting protein; KRAB-associated protein 1; KRAB-interacting p; KRAB-interacting protein 1; Krip1; KRIP-1; MommeD9; Nuclear corepressor KAP-1; PPP1R157; protein phosphatase 1, regulatory subunit 157; RING finger protein 96; RING-type E3 ubiquitin transferase TIF1-beta; RNF96; TF1B; tif1 beta; Tif1b; Tif1beta; TIF1-beta; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1, beta; transcriptional intermediary factor 1-beta; Trim28; tripartite motif; tripartite motif containing 28; tripartite motif protein 28; tripartite motif-containing 28; tripartite motif-containing protein 28
• Gene Symbols: TRIM28
• Primary or Secondary: Primary
• Purification Method: Purified