Thermo Scientific™ Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.9%

Description

CAS: 77-86-1 | C4H11NO3 | 121.136 g/mol

Used as a biological buffer substance in the pH range 7—9; pKa = 8.3 at 20°C; pKa = 7.82 at 37°C. Used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0-9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins. Used in chemical manufacturing (surface-active agents, vulcanization accelerators, and pharmaceuticals), as an emulsifying agent (cosmetics, mineral oil and paraffin wax emulsions, leather dressings, textile specialties, polishes, cleaning compounds, and soluble oils), and as an absorbent for acidic gases, biological buffer, acidimetric standard, and therapeutic alkalinizing agent. Used in buffer systems for the freezer storage of sperm, to regulate pH in the transport of live aquatic species, cell culture media, and anti-inflammatory drugs.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Notes
Store away from oxidizing agents. Keep the container tightly closed and place it in a cool, dry and well ventilated condition.

Specifications

Chemical Identifiers

• CAS: 77-86-1
• Molecular Formula: C4H11NO3
• Molecular Weight (g/mol): 121.136
• MDL Number: MFCD00004679
• InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N
• Synonym: 2-Amino-2-hydroxymethyl-1,3-propanediol; TRIS
• PubChem CID: 6503
• ChEBI: CHEBI:9754
• IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol
• SMILES: C(C(CO)(CO)N)O

• Melting Point: 168°C to 172°C
• Boiling Point: 219°C to 220°C (10mmHg)
• Color: White
• Density: 1.353
• Physical Form: Crystalline Powder
• Quantity: 500 g
• Beilstein: 741883
• Merck Index: 14,9772
• Solubility Information: Not miscible or difficult to mix in water. Soluble in ethylene glycol (79.1mg/ml),methanol (26mg/ml),anhydrous ethanol (14.6mg/ml),95% ethanol (22mg/ml),DMF (14mg/ml),acetone (2mg/ml),ethyl acetate (0.5mg/ml),olive oil (0.4mg/ml),chloroform (0.05mg/ml),and alkaline solution.
• Formula Weight: 121.14
• Percent Purity: 99.9%
• Grade: Electrophoresis
• Chemical Name or Material: Tris(hydroxymethyl)aminomethane

Safety and Handling

Hazard Category	Warning
Hazard Statement	GHS H Statement H315-H319-H335 Causes skin irritation. Causes serious eye irritation. May cause respiratory irritation.
Precautionary Statement	GHS P Statement P261-P280-P305+P351+P338-P304+P340-P405-P501a Avoid breathing dust/fume/gas/mist/vapors/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. IF INHALED: Remove to fresh air and keep at rest in a position comfortable for breathing. Store locked up. Dispose of contents/container in accordance with local/regional/national/international regulations.
EINECSNumber	201-064-4
RTECSNumber	TY2900000
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only