TRIS, MP Biomedicals™

Description

TRIS, Molecular biology grade, >99.8%, White Crystalline Powder

• Tris may be used by itself as a buffer or as a component of mixed buffer formulations e.g. reaction buffers for restriction enzymes or nucleic acid modifying enzymes.
• Tris salts are used in protein crystallization at various pH values.
• The use of low-ionic strength Tris buffers in the formation of intermediate filaments of lamin from Caenorhabditis elegans has been described.
• Tris has been utilized in studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences.
• The use of Tris in capillary electrochromatography and UV analysis of tocopherols and tocotrienols has been reported.
• Tris is an established basimetric standard and buffer used in biochemistry and molecular biology

Specifications

Chemical Identifiers

• CAS: 77-86-1
• Molecular Formula: C4H11NO3
• Molecular Weight (g/mol): 121.14
• InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N
• Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris-(hydroxymethyl) aminomethane, Trometamol

• Melting Point: 167°C to 172°C (literature)
• Boiling Point: 219°C to 220°C/10 mmHg(literature)
• Color: White
• pH: 10.5 to 11.5 (0.5 M aq. soln., 20°C)
• Physical Form: Crystalline Powder
• Quantity: 500 g
• Beilstein: 741883
• Solubility Information: Soluble in water (550mg/mL), ethyleneglycol (79.1mg/mL), methanol (26mg/mL), anhydrous ethanol (14.6mg/mL), 95% ethanol (22mg/mL), DMF (14mg/mL), acetone (2mg/mL), ethyl acetate (0.5mg/mL), olive oil (0.4mg/mL), chloroform (0.05mg/mL), and alkaline solution.
• Grade: >99.8%

Safety and Handling

RTECSNumber	TY2900000
storageNote1	Store at Room temperature(15-30°C), desiccate.