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Description
- Tris may be used by itself as a buffer or as a component of mixed buffer formulations e.g. reaction buffers for restriction enzymes or nucleic acid modifying enzymes.
- Tris salts are used in protein crystallization at various pH values.
- The use of low-ionic strength Tris buffers in the formation of intermediate filaments of lamin from Caenorhabditis elegans has been described.
- Tris has been utilized in studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences.
- The use of Tris in capillary electrochromatography and UV analysis of tocopherols and tocotrienols has been reported.
- Tris is an established basimetric standard and buffer used in biochemistry and molecular biology
Specifications
Specifications
| Melting Point | 167°C to 172°C (literature) |
| Boiling Point | 219°C to 220°C/10 mmHg(literature) |
| Color | White |
| pH | 10.5 to 11.5 (0.5 M aq. soln., 20°C) |
| Physical Form | Crystalline Powder |
| Quantity | 500 g |
| Beilstein | 741883 |
| Solubility Information | Soluble in water (550mg/mL), ethyleneglycol (79.1mg/mL), methanol (26mg/mL), anhydrous ethanol (14.6mg/mL), 95% ethanol (22mg/mL), DMF (14mg/mL), acetone (2mg/mL), ethyl acetate (0.5mg/mL), olive oil (0.4mg/mL), chloroform (0.05mg/mL), and alkaline solution. |
| Grade | >99.8% |
Safety and Handling
| RTECSNumber | TY2900000 |
| storageNote1 | Store at Room temperature(15-30°C), desiccate. |
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