Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Invitrogen™ TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System

Catalog No. A33252
Encompass_Preferred
Click to view available options
Quantity:
100 Preps
200 Preps

Includes

One bottle of TRIzol LS Reagent 100 mL and one bag of 100 Phasemaker Tubes

This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.

This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.

The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.

• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments

Warranty and Services

One year after receiving

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage • 100 mL TRIzol LS Reagent; room temperature
• 100 Phasemaker Tubes; room temperature

Isolation Technology Organic Extraction
Purification Time 1 hr.
Elution Volume 20 to 50 μL
Sample Type Liquid Samples (e.g. Serum)
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Quantity 100 Preps
Shipping Condition Room Temperature
Starting Material Amount 0.25 mL per 0.75 mL of TRIzol™ LS Reagent
Yield Tissue: ≤10 μg per mg
Cells: ≤15 μg per 106 cells
Show More Show Less
WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.