Invitrogen™ TRIzol™ LS Reagent and Phasemaker™ Tubes Complete System

Description

Includes

One bottle of TRIzol LS Reagent 100 mL and one bag of 100 Phasemaker Tubes

This system includes both TRIzol™ LS Reagent and Invitrogen™ Phasemaker™ Tubes.

This is a complete system that includes the TRIzol™ Reagent and the Phasemaker™ Tubes. The TRIzol Reagent offers superior lysis capability, even with difficult sample types. Invitrogen™ Phasemaker Tubes contain a polymer that clearly separates and completely isolates the upper aqueous phase of the TRIzol mix from the organic phase underneath, making very easy to pipette off the upper phase containing the RNA.

The polymer works on density gradient, as it is heavier than the aqueous phase, but lighter that the organic phase; after centrifugation it positions itself between these two layers. It creates a tight seal between the two phases, making it very easy to pipette off the aqueous phase without the risk of carrying over contaminants from the organic layer. Because this, Invitrogen Phasemaker Tubes allow you to recover the whole aqueous phase, increasing yield up to 30%. It is an inert polymer that does not interfere with downstream experiments such as PCR. It works with fresh or frozen samples (tissue or blood). It also works with tissue samples treated previously with RNAlater. Invitrogen Phasemaker Tubes come already pre-dispensed. The tube size is 2 mL.

• Complete System includes TRIzol and Phasemaker Tubes
• Phasemaker Tubes are pre-dispensed in 2 mL tubes
• Creates a tight seal between the aqueous and the organic phases
• Easier to pipette off the whole aqueous phase
• Increases yield up to 30%
• RNA integrity is not compromised
• The seal stays for longer periods of time, allowing to increase the number of samples per batch
• Inert, does not interfere with downstream experiments

Warranty and Services

One year after receiving

Specifications

• Content And Storage: • 100 mL TRIzol LS Reagent; room temperature
  • 100 Phasemaker Tubes; room temperature
  
  
• Isolation Technology: Organic Extraction
• Purification Time: 1 hr.
• Elution Volume: 20 to 50 μL
• Sample Type: Liquid Samples (e.g. Serum)
• Final Product Type: Total RNA
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
• Quantity: 100 Preps
• Shipping Condition: Room Temperature
• Starting Material Amount: 0.25 mL per 0.75 mL of TRIzol™ LS Reagent
• Yield: Tissue: ≤10 μg per mg
  Cells: ≤15 μg per 10⁶ cells

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.