Tropomyosin-1, Rat anti-Mouse, Human, Clone: 7F8B8, Millipore Sigma™

Rat Monoclonal Antibody

Supplier:  MilliporeSigma MABT136525UL

Catalog No. MABT136525

Description

Anti-Tropomyosin-1, clone 7F8B8, Cat. No. MABT1365, is a rat monoclonal antibody that detects Tropomyosin-1 and has been tested for use in Immunocytochemistry and Western Blotting.

Tropomyosin alpha-1 chain (UniProt: P09493; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the TPM1 (also known as C15orf13, TMSA) gene (Gene ID: 7168) in human. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Ten different isoforms of Tropomyosin-1 have been described that are produced by alternative splicing.

Specifications

• Antigen: Tropomyosin-1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: TPM1;C15orf13;TMSA
• Immunogen: KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.
• Quantity: 25 μL
• Research Discipline: Cell Structure
• Gene ID (Entrez): NP_000357.3
• Target Species: Mouse, Human
• Form: Purified

• Applications: Immunocytochemistry, Western Blot
• Clone: 7F8B8
• Dilution: Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
• Gene Alias: Tropomyosin alpha-1 chain;Alpha-tropomyosin;Tropomyosin-1
• Host Species: Rat
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG2a κ