Invitrogen™ Troponin I Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA537897

Catalog No. PIPA537897

Description

This antibody is predicted to react with pig and cow based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

Specifications

• Antigen: Troponin I
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TNNI1
• Gene Alias: SSTNI; TNN1
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 21952, 7135
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P19237, Q9WUZ5
• Gene Symbols: TNNI1
• Immunogen: Peptide with sequence PEVERKPKITASRK-C, from the N-terminus of TNNI1 (aa 2-15).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG