TRPC6 Monoclonal Antibody (3F2.H10.F2), Rockland™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-199-0579	Rockland Immunochemicals 200301B59	100 μg
50-199-0580	Rockland Immunochemicals 200301B59S	25 μL

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for human TRPC6 protein. A BLAST analysis was used to suggest cross-reactivity with TRPC6 from chimpanzee based on 100% homology with the immunizing sequence. Cross-reactivity with TRPC6 from other sources has not been determined.

Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of the Trpc6 channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Further, Trpc6 is a member of the mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the 'canonical TRP' (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, Trpc6 acts as a non-selective store-independent receptor-activated cation channel. Trpc6 is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.

Specifications

• Antigen: TRPC6
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Alias: AV025995; Calcium entry channel; FLJ11098; FLJ14863; focal segmental glomerulosclerosis 2; FSGS2; HGNC:12338; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; Short transient receptor potential channel 6; transient receptor potential cation channel subfamily C member 6; transient receptor potential cation channel, subfamily C, member 6; Transient receptor protein 6; Trp6; TRP-6; trp6A; TRPC6; Trrp6
• Gene Symbols: Trpc6
• Isotype: IgG1 κ
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Gene: Trpc6

• Clone: 3F2.H10.F2
• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Chimpanzee, Human
• Gene Accession No.: Q9Y210
• Gene ID (Entrez): 451503, 7225
• Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.
• Primary or Secondary: Primary
• Product Type: Antibody