Invitrogen™ TRPC6 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA529848

Catalog No. PIPA529848

Description

Recommended positive controls: NT2D1, MCF-7, A549, A549 membrane fraction extract. Predicted reactivity: Mouse (85%), Rat (88%), Bovine (91%), Guinea pig (91%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Trpc6 forms a receptor-activated calcium channel in the cell membrane. The Trpc6 channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of the Trpc6 channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in the Trpc6 gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). Further, Trpc6 is a member of the mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the 'canonical TRP' (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC6/6/7 subfamily can be activated by diacylglycerol (DAG) analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. When expressed in transfected cells, Trpc6 acts as a non-selective store-independent receptor-activated cation channel. Trpc6 is activated by DAG in a PKC-independent manner and is insensitive to IP3 activation. There is increasing evidence that Trpc6 encodes endogenous DAG-activated receptor-operated cation channels in vivo. Diseases associated with TRPC6 include Glomerulosclerosis, Focal Segmental, 2 and Focal Segmental Glomerulosclerosis.

Specifications

• Antigen: TRPC6
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Trpc6
• Gene Alias: AV025995; bZ1P14.9; calcium entry channel; FLJ11098; FLJ14863; focal segmental glomerulosclerosis 2; FSGS2; HGNC:12338; LLHWJM002; LLHWJM003; LLHWJM004; mtrp6; Short transient receptor potential channel 6; short transient receptor potential channel 6; short transient receptor potential channel 6a; si:rp71-1p14.9; transient receptor potential cation channel subfamily C member 6; transient receptor potential cation channel subfamily c member 6a; transient receptor potential cation channel TRPC6; transient receptor potential cation channel, subfamily C, member 6; transient receptor potential cation channel, subfamily C, member 6a; transient receptor potential channel 6; transient receptor potential channel subfamily C member 6; transient receptor protein 6; TRP6; TRP-6; trp6A; TRPC6; trpc6a; Trrp6
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7225
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9Y210
• Gene Symbols: Trpc6
• Immunogen: Recombinant protein encompassing a sequence within the Intracellular domain of human TRPC6. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov