Invitrogen™ TRPM7 Monoclonal Antibody (S74), APC

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545423

Catalog No. PIMA545423

Description

1 μg/mL of MA5-45423 was sufficient for detection of TrpM7 in 10 μg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

Specifications

• Antigen: TRPM7
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.64mM phosphate with 0.5M EDTA and no preservative; pH 7.4
• Gene Accession No.: Q923J1, Q925B3, Q96QT4
• Gene Symbols: Trpm7
• Immunogen: Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Western Blot, Immunocytochemistry, Western Blot
• Clone: S74
• Conjugate: APC
• Gene: Trpm7
• Gene Alias: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250; Ltpr7; LTRPC ion channel family member 7; LTRPC7; LTrpC-7; LTrpC7 {ECO:0000250; PubMed:11161216}; RGD:620053}; tr; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; Trpm7; trpm7 {ECO:0000312; TRPPLIK; Trp-plik; TRP-PLIK {ECO:0000303; UniProtKB:Q96QT4}
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 54822, 58800, 679906
• Content And Storage: 4°C
• Form: Liquid

Safety and Handling

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