MilliporeSigma™ TRRAP, Mouse, Unlabeled, Clone: 2TRR-1B3,

Catalog No.	Quantity	Conjugate
MABE1008MI	100 μL	Unconjugated

Catalog No. MABE1008MI Supplier MilliporeSigma™ Supplier No. MABE1008

Description

Mouse Monoclonal Antibody

Platelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human. Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation. PF4 binds heparin with high affinity (Kd ∽4-20 nM) via its heparin-binding domain (a.a. 92-98) and neutralizes the anti-thrombin activity of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B. In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation. Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance. PF4 is synthesized with a signal peptide sequence (a.a. 1-31) that is removed posttranslationally to produce the mature PF4 (a.a. 32-101), additional proteolytic cleavage yields the PF4 short form (a.a. 48-101).

Specifications

• Antigen: TRRAP
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 2TRR-1B3
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Mouse monoclonal IgG2aκ ascites without preservatives.
• Gene: TRRAP, PAF400
• Gene Accession No.: Q9Y4A5
• Host Species: Mouse
• Immunogen: Ovalbumin-conjugated linear peptide corresponding to a sequence from the TP53-interacting region of human TRRAP.
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Epigenetics & Nuclear Function
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001231509
• Test Specificity: The immunogen sequence is present in both spliced isoforms of human TRRAP reported by UniProt (Q9Y4A5).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Ascites
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.