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Trypsin, 1-250 from Porcine Pancreas , MP Biomedicals, LLC

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Description

Trypsin, 1-250 from Porcine Pancreas has an activity >250 USP u/mg. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.

  • The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture
  • The 1:250 designation indicates the tryptic activity based on standardized testing
  • This ratio indicates that one part trypsin will digest 250 parts casein in the USP activity assay
  • Trypsin is composed of two subunits, a-trypsin and b-trypsin. a-Trypsin is composed of two peptide chains and b-trypsin is composed of one chain
  • Trypsin is used to re-suspend cells adherent to the cell culture dish wall during the process of harvesting cells
  • Trypsin can also be used to dissociate dissected cells
  • Trypsin can be used to break down casein in breast milk
  • Trypsin is commonly used in biological research during proteomics experiments to digest proteins into peptides for mass spectrometry analysis
  • Trypsin can also be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form
  • One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 7-7-9002
Molecular Formula C35H47N7O10
Molecular Weight (g/mol) 725.80
InChI Key WGWZNYKOUXOZTC-UHFFFAOYNA-N
Synonym tryptase, trypsin, tryptase from human lung, tryptase from human lung, buffered aqueous solution, ≥5 units/mg protein, acetic acid; tert-butyl n-2s-1-2s-1-2s-5-diaminomethylideneamino-1-5-methyl-2-oxochromen-7-yl amino-1-oxopentan-2-yl amino-3-hydroxy-1-oxopropan-2-yl amino-1-oxo-3-phenylpropan-2-yl carbamate
PubChem CID 5311489

Specifications

Color White
pH ∼8.0
Packaging Glass
Quantity 20 g
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