Invitrogen™ TPH1 Polyclonal Antibody

Sheep Polyclonal Antibody

Supplier:  Invitrogen™ PA14678

Catalog No. PA14678

Description

This antibody is predicted to react with wide variety of mammals based on 100% sequence homology, however it does not recognize TPH in rabbit tissues. This antibody is specific for the ~55 kDa TPH protein in Western blots of human dorsal Raphe nucleus. Immunolabeling has been demonstrated in rat brain extracts.

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Specifications

• Antigen: TPH1
• Classification: Polyclonal
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: P09810, P17290, P17752
• Gene Symbols: TPH1
• Immunogen: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Rat, Rabbit
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunohistochemistry, Immunohistochemistry (Frozen), Western Blot
• Conjugate: Unconjugated
• Gene: TPH1
• Gene Alias: indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; Tph1; TPH2; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)
• Host Species: Sheep
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100009100, 100101558, 24848, 7166
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid