Invitrogen™ TSC1 Polyclonal Antibody

Catalog No.	Quantity
PIPA580193	100 μg

Catalog No. PIPA580193 Supplier Invitrogen™ Supplier No. PA580193

Description

Rabbit Polyclonal Antibody

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Rat Skeletal Muscle Tissue, Rat Heart Tissue, Rat Brain Tissue, Rat Lung Tissue, 293T whole cell, HELA whole cell, HT1080 whole cell, SMMC whole cell. IHC: Human Mammary Cancer Tissue, Rat Brain Tissue.

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Specifications

• Antigen: TSC1
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 500 μg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide, 0.05mg thimerosal
• Gene: TSC1
• Gene Accession No.: Q92574, Q9EP53, Q9Z136
• Gene Alias: hamartin; HGNC:12362; Kiaa0243; LAM; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; TSC; Tsc1; tuberous sclerosis 1; tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor
• Gene Symbols: TSC1
• Host Species: Rabbit
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin (1150-1164aa QLHIMDYNETHHEHS).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 60445, 64930, 7248
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG