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  6. TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Catalog No. 111021850 Shop All MilliporeSigma Supelco Products
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Diameter (Metric):
5 mm
7.5 mm
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Catalog No. Diameter (Metric)
11-102-1850 5 mm
11-102-1830 7.5 mm
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Catalog No. 11-102-1850 Supplier MilliporeSigma Supelco Supplier No. 814449
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Description

High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins.

  • High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
  • Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions
  • This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines
  • Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers
  • Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine
  • Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris
  • Resin Adsorption Capacity: 40 μmol/mL
TRUSTED_SUSTAINABILITY

Specifications

Diameter (Metric) 5 mm
Flow Rate 1.2 mL/min
Length (Metric) 5 cm
Max. Pressure 30 bar
Particle Size 10 μm
pH 2 to 9
Solvents ≤ 20% Organic Solvent
Temperature >0°C
Stationary Phase M-Aminophenyl Boronate
Product Line TSKgel
Sample Type Proteins
Matrix M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
Material Glass
Technique Affinity
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TSKgel is a registered trademark of Tosoh Corporation

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