TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Description

High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins.

• High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
• Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions
• This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines
• Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers
• Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine
• Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris
• Resin Adsorption Capacity: 40 μmol/mL

Specifications

• Diameter (Metric): 5 mm
• Flow Rate: 1.2 mL/min
• Length (Metric): 5 cm
• Max. Pressure: 30 bar
• Particle Size: 10 μm
• pH: 2 to 9
• Solvents: ≤ 20% Organic Solvent
• Temperature: >0°C
• Stationary Phase: M-Aminophenyl Boronate
• Product Line: TSKgel
• Sample Type: Proteins
• Matrix: M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
• Material: Glass
• Technique: Affinity

TSKgel is a registered trademark of Tosoh Corporation