Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Chromatography Columns and Cartridges
  4. HPLC Columns and Cartridges
  5. HPLC Columns
  6. TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

Catalog No. 111021849 Shop All MilliporeSigma Supelco Products
Change view
Click to view available options
Diameter (Metric):
5 mm
7.5 mm
21.5 mm
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Diameter (Metric)
11-102-1849 5 mm
11-102-1816 7.5 mm
11-102-1817 21.5 mm
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. 11-102-1849 Supplier MilliporeSigma Supelco Supplier No. 814440
Only null left
Add to Cart
Add to Cart

Description

Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, loaded with divalent metal ions by chelation.

  • High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
  • Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+
  • Column is loaded with divalent metal ions by chelation
  • Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH
  • Retained compounds can be eluted with buffer containing imidazole or glycine
  • A decreasing pH gradient can be used for elution purposes as well
  • For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes
  • Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides
TRUSTED_SUSTAINABILITY

Specifications

Diameter (Metric) 5 mm
Flow Rate 1 mL/min
Length (Metric) 5 cm
Max. Pressure 20 bar
Particle Size 10 μm
pH 2 to 12
Solvents ≤ 20% Organic Solvent
Temperature ≤45°C
Stationary Phase Iminodiacetic Acid
Product Line TSKgel
Matrix Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
Material Glass
Technique Affinity
Show More Show Less

TSKgel is a registered trademark of Tosoh Corporation

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.