TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™

Description

Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, loaded with divalent metal ions by chelation.

• High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins
• Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+
• Column is loaded with divalent metal ions by chelation
• Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH
• Retained compounds can be eluted with buffer containing imidazole or glycine
• A decreasing pH gradient can be used for elution purposes as well
• For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes
• Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides

Specifications

• Diameter (Metric): 5 mm
• Flow Rate: 1 mL/min
• Length (Metric): 5 cm
• Max. Pressure: 20 bar
• Particle Size: 10 μm
• pH: 2 to 12
• Solvents: ≤ 20% Organic Solvent
• Temperature: ≤45°C
• Stationary Phase: Iminodiacetic Acid
• Product Line: TSKgel
• Matrix: Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
• Material: Glass
• Technique: Affinity

TSKgel is a registered trademark of Tosoh Corporation