TSKgel™ NH2-100 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Description

TSKgel™ NH2-100 amino columns expand the range of TSKgel™ columns for hydrophilic interaction chromatography (HILIC). Offering a different selectivity from the well known TSKgel™ Amide-80 columns, these novel amino-bonded phase columns stand out by providing much improved chemical stability, a prerequisite for achieving reproducible and reliable results.

TSKgel™ NH2-100 columns are unique in that the bonded phase ligand not only, as expected, has a terminal primary amino group, but that the spacer also incorporates secondary as well as tertiary amino groups. Anionic compounds are retained on the column by ionic interaction. This allows for the use of gradients in salt concentration, in addition to gradient elutions with acetonitrile. Since the TSKgel™ NH2-100 columns have cationic sites, the columns can be used as mixed mode columns under some conditions. TSKgel™ NH2-100 columns are well suited for the analysis of all types of hydrophilic compounds, including carbohydrates and peptides. Due to a high ligand density and large surface area, these columns show stronger retention of polar compounds than TSKgel™ Amide-80 columns.

Order Info

Shipped in 85% acetonitrile - 15% water.

Specifications

• Diameter (Metric): 2 mm
• Flow Rate: 0.2 mL/min.
• Length (Metric): 5 cm
• Max. Pressure: 150 bar
• Particle Size: 3 μm
• pH: 2.0 - 7.5
• Solvents: 0 to 100% organic solvent
• Surface Area: 450 m2/g
• Temperature: 10°C to 50°C
• USP Type: L8
• Stationary Phase: Amine
• Product Line: TSKgel
• Matrix: Silica Particle Platform
• Phase: Normal Phase, HILIC, Ion Exchange
• Material: Stainless Steel Hardware

TSKgel is a registered trademark of Tosoh Corporation