TSKgel™ Phenyl-5PW RP HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

Description

TSKgel™ Phenyl-5PW RP columns are filled with 10 micron porous polymethacrylate particles, the surface of which is covered with a high density of phenyl groups that causes proteins to be retained in a low salt buffer mobile phase and requiring a solvent gradient for their elution, as is the protocol in reversed phase chromatography.

In contrast, TSKgel™ Phenyl-5PW has such a low density of phenyl groups on the surface that proteins can only be retained at high salt concentration and eluted by a decreasing salt gradient, which, of course, is the protocol used in hydrophobic interaction chromatography. TSKgel™ Phenyl-5PW RP columns can withstand rigorous cleaning with either acid or base. The 1000 Å pore size accommodates globular protein samples up to 1,000,000 Da.

Order Info

Shipped in 30% methanol - 70% water.

Specifications

• Diameter (Metric): 4.6 mm
• Flow Rate: 1.2 mL/min.
• Length (Metric): 7.5 cm
• Ligand Type: 1 meq/mL ligand density (estimate)
• Max. Pressure: 30 bar
• Particle Size: 10 μm
• pH: 2.0 - 12
• Pore Size: 1000 Å
• Solvents: 0 to 100% organic solvent
• Temperature: 4°C to 60°C
• Stationary Phase: Phenyl
• Product Line: TSKgel
• Matrix: Polymer Particle Platform
• Phase: Reversed Phase
• Material: Stainless Steel

TSKgel is a registered trademark of Tosoh Corporation