TTF-1/ NKX2-1 Antibody (NX2.1/690) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP247772A	Novus Biologicals NBP2477720.1MG	0.1 mg
NBP247772B	Novus Biologicals NBP2477720.2MG	0.2 mg

Description

TTF-1 / NKX2-1 Monoclonal specifically detects TTF-1 / NKX2-1 in Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.

Specifications

• Antigen: TTF1 / NKX2.1
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Membrane Trafficking and Chaperones, Tumor Biomarkers
• Formulation: PBS with No Preservative
• Gene ID (Entrez): 7080
• Immunogen: Recombinant TTF-1 protein
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 40 kDa

• Clone: NX2.1/690
• Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat
• Gene Alias: benign chorea, Homeobox protein NK-2 homolog A, homeobox protein Nkx-2.1, NK2 homeobox 1, NK-2 homolog A, NKX2ANKX2.1, Thyroid nuclear factor 1, Thyroid transcription factor 1BHC, TITF1TEBP, TTF1, TTF1BCH, TTF-1NK-2
• Gene Symbols: NKX2-1
• Isotype: IgG1 κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.