Invitrogen™ TTLL10 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531631

Catalog No. PIPA531631

Description

Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7, NIH-3T3. Predicted reactivity: Mouse (82%), Rat (82%), Rhesus Monkey (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The TTLL10 gene encodes a protein that functions as a polyglycylase, playing an important role in the modification of nucleosome assembly protein 1 (NAP1) both in vivo and in vitro. This enzymatic activity is dependent on the presence of ATP and is abolished in mutant forms where the glutamate in the active site is replaced by valine. TTLL10 specifically incorporates glycine into recombinant NAP1 at Glu residues 359 and 360. This modification process likely has implications for protein interactions and cellular processes involving NAP1. Additionally, TTLL10 can perform tubulin glycylation when co-expressed with TTLL8, suggesting a complex interplay between these enzymes in cellular microtubule dynamics.

Specifications

• Antigen: TTLL10
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TTLL10
• Gene Alias: 4833412E22Rik; 4930595O22Rik; AU022799; BC042775; Inactive polyglycylase TTLL10; protein polyglycylase TTLL10; Ttll10; Ttll5; tubulin tyrosine ligase like 10; tubulin tyrosine ligase-like family member 10; tubulin tyrosine ligase-like family, member 10; tubulin tyrosine ligase-like family, member 5; tubulin--tyrosine ligase-like protein 10
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 254173, 330010
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: A4Q9F3, Q6ZVT0
• Gene Symbols: TTLL10
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TTLL10. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG