MP Biomedicals™ Tubulin, Purified from Bovine Brain

Tubulin, Purified from Bovine Brain is a heterodimer composed of alpha- and beta-tubulin of 55 kDa (molecular weight ∼110 kDa).

Supplier:  MP Biomedicals™ 08771121

Catalog No. 08-771-121

Description

• Purified bovine brain tubulin protein supplied in liquid form at 10 mg/mL in 80 mM PIPES buffer containing 1 mM EGTA, 1 mM GTP, pH 6.8 and 10% glycerol.
• It is a heterodimer composed of α- and β- tubulin of 55 kDa (molecular weight ≅110 kDa).
• Polymerization occurs forming microtubules of 25 nm in diameter and average 1650 heterodimers per μm in length.
• One unit of tubulin is defined as 5.0 mg of purified protein (as determined by the method of Bradford: Bradford, M., Analytical Biochemistry 72, 248, 1976).

Specifications

• Description:

  Preparation Method

  One unit of tubulin in G-PEM buffer pH 6.8 plus 10% glycerol was incubated at 35°C in a cuvette. The optical density at 340 nm was taken at time intervals. A value of 1.0 OD unit indicates that >97% of the tubulin has polymerized. The polymerization reaction was performed in duplicate.

• Quantity: 1 mg
• Test Specificity: When tubulin at a concentration of 1.0 unit/mL (in G-PEM buffer plus 10% glycerol [v/v]) is incubated at 35°C for 30 minutes an increase in optical density (OD340nm) from 0.0 to 1.0 is observed. This corresponds to a total microtubule (MT) polymer mass of >4.8 mg/mL. The mean length of the microtubule population under these conditions is approximately 10 μm as measured by immunoflourescence microscopy and the average number of MTs per ml is 1.0 x 10¹².
• Form: Liquid

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use.