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Description
Western blot analysis of PA1-178 detects an ~16-18 kDa protein in Tluc expressing cells. PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.
TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family. The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression. Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light. Tluc is smaller and brighter than other commonly used luciferases, including firefly and Renilla. The intense bioluminescence greatly enhances sensitivity of Tluc luciferase-based assays, enabling detection of very minute amounts of luciferase (signal) in very sensitive assays such as protein interaction qIP assays (Prod # 82032, 82033, 82035, 82036).
Specifications
| TurboLuc luciferase | |
| Polyclonal | |
| Unconjugated | |
| TurboLuc luciferase synthetic | |
| Purified recombinant TurboLuc luciferase expressed in E. coli. | |
| 100 μg | |
| Primary | |
| -20°C | |
| Liquid |
| Western Blot | |
| 1 mg/mL | |
| PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide | |
| Rabbit | |
| Protein G | |
| RUO | |
| Luciferase | |
| Antibody | |
| IgG |
Safety and Handling
WARNING: Cancer - www.P65Warnings.ca.gov
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