Twist-1 Mouse anti-Human, Clone: 927403, R&D Systems™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MAB6230SP	R&D Systems MAB6230SP	25 ug
MAB6230	R&D Systems MAB6230	100 ug

Description

Twist-1 (Twist-related protein 1; also H-Twist and bHLHa38) is a 25-27kDa class B member of the bHLH transcription factor family of proteins. It is widely expressed in embryo, and select adult cells such as white adipocytes. In fat, Twist-1 induces fatty acid oxidation via PGC-1α and CPT-1, and promotes MCP-1 and TNF-α secretion by adipocytes. In epithelium, Twist-1 dysregulation represses E-Cadherin and induces N-Cadherin expression, resulting in an epithelial-to-mesenchymal transition that can lead to cancer. Human Twist-1 is 202 amino acids (aa) in length. It contains a bHLH domain (aa 109-164) with an embedded DNA-binding motif. Twist-1 forms homodimers, and heterodimerizes with TCF3, HAND 1 and HAND 2. Two distinct mutations exist that may impact its dimerization pattern. There is one seven aa insertion after Ile135, and a second seven aa insertion after Pro139. A third unrelated variant shows a four Gly insert after Gly92. Full-length human Twist-1 shares 98% aa identity with mouse Twist-1.

Specifications

• Antigen: Twist-1
• Format: Purified
• Applications: CyTOF, Flow Cytometry, Western Blot
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO

• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Clone: 927403
• Conjugate: Unconjugated
• Isotype: IgG1
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.