Invitrogen™ Twist1/Twist2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA578211

Catalog No. PIPA578211

Description

Positive Control: Raji, THP-1, HL-60, TWIST1 transfected 293T lysates, human TWIST1-transfected 293T, Jurkat, OECM1, CL1-5, Toledo, SU-DHL-5, HT, DB, RL95-2, MDA-MB-231 Predicted Reactivity: Japanese Medaka (95%), Xenopus laevis (97%), Chicken (100%), Chimpanzee (100%), Bovine (100%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Twist1/Twist2 encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. Twist1/Twist2 is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.

Specifications

• Antigen: Twist1/Twist2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TWIST1
• Gene Alias: AA960487; ACS3; AMS; BBRSAY; B-HLH DNA binding protein; bHLHa38; BHLHA39; BPES2; BPES3; charlie chaplin; class A basic helix-loop-helix protein 38; Class A basic helix-loop-helix protein 39; CRS; CRS1; CSO; dermis expressed 1; dermis-expressed protein 1; DERMO1; Dermo-1; dermo-1 protein; FFDD3; H-twist; MGC117334; M-Twist; Pde; pdt; Pluridigite; polydactyly EMS; SCS; SETLSS; Ska>m10Jus>; Ska>sup>m10Jus>/sup>; Ska10; Twist; twist basic helix-loop-helix transcription factor 1; twist basic helix-loop-helix transcription factor 2; twist family bHLH transcription factor 1; twist family bHLH transcription factor 2; twist gene homolog 1; twist homolog 1; twist homolog 2; TWIST homolog of drosophila; Twist1; Twist2; twist-related bHLH protein Dermo1; twist-related protein 1; Twist-related protein 2
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 117581, 13345, 22160, 59327, 7291, 85489
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.67 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P26687, P97831, Q15672, Q8WVJ9, Q9D030
• Gene Symbols: TWIST1, TWIST2
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Drosophila
• Product Type: Antibody
• Isotype: IgG