Invitrogen™ TYW1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA530677

Catalog No. PIPA530677

Description

Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (88%), Rat (89%), Zebrafish (84%), Xenopus laevis (87%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions.

Specifications

• Antigen: TYW1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: TYW1
• Gene Alias: Radical S-adenosyl methionine and flavodoxin domain-containing protein 1; radical S-adenosyl methionine and flavodoxin domains 1; RGD1310420; RSAFD1; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase; S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1; tRNA wybutosine-synthesizing protein 1 homolog; tRNA-yW synthesizing protein 1 homolog; tRNA-yW synthesizing protein 1 homolog (S. cerevisiae); tRNA-yW synthesizing protein 1 homolog A; tRNA-yW-synthesizing protein; TYW1; TYW1A; YPL207W
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 55253
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.92 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9NV66
• Gene Symbols: TYW1
• Immunogen: Recombinant protein fragment corresponding to a region within amino acids 480 and 662 of Human TYW1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov