Invitrogen™ UBE2E3 Monoclonal Antibody (OTI7E8)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA526364

Catalog No. PIMA526364

Description

UBE2E3 Monoclonal Antibody for Western Blot, ICC/IF, IHC (P), Flow

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.The first step requires the ATP-dependent activation of the Ub C-terminusand the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. A wide range of enzymes facilitate in the proteolytic Ub pathway including UBE2E3, also designated UBCH9, which catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. UBE2E3 may also be involved in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in a IPO11-dependent manner. It is ubiquitously expressed at low levels and is highly expressed in skeletal muscle.

Specifications

• Antigen: UBE2E3
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: P52483, Q969T4
• Gene Symbols: UBE2E3
• Immunogen: Full length human recombinant protein of UBE2E3 produced in E.coli.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Canine, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: OTI7E8
• Conjugate: Unconjugated
• Gene: UBE2E3
• Gene Alias: E2 ubiquitin-conjugating enzyme E3; Ubce4; UBCH9; UbcM2; UBE2E3; Ubiquitin carrier protein E3; ubiquitin conjugating enzyme E2 E3; ubiquitin conjugating enzyme E2E 3; ubiquitin-conjugating enzyme 4; ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3; ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2E 3, UBC4/5 homolog; ubiquitin-protein ligase E3
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10477, 22193, 295686, 612823
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid