Invitrogen™ UBE2E3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531122

Catalog No. PIPA531122

Description

Recommended positive controls: mouse muscle. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (93%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.The first step requires the ATP-dependent activation of the Ub C-terminusand the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. A wide range of enzymes facilitate in the proteolytic Ub pathway including UBE2E3, also designated UBCH9, which catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. UBE2E3 may also be involved in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in a IPO11-dependent manner. It is ubiquitously expressed at low levels and is highly expressed in skeletal muscle.

Specifications

• Antigen: UBE2E3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE2E3
• Gene Alias: E2 ubiquitin-conjugating enzyme E3; Ubce4; UBCH9; UbcM2; UBE2E3; Ubiquitin carrier protein E3; ubiquitin conjugating enzyme E2 E3; ubiquitin conjugating enzyme E2E 3; ubiquitin-conjugating enzyme 4; ubiquitin-conjugating enzyme E2 E3; Ubiquitin-conjugating enzyme E2-23 kDa; ubiquitin-conjugating enzyme E2E 3; ubiquitin-conjugating enzyme E2E 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2E 3, UBC4/5 homolog; ubiquitin-protein ligase E3
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10477, 22193
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.63 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P52483, Q969T4
• Gene Symbols: UBE2E3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 207 of Human UBE2E3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov