Invitrogen™ UBE2O Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA5142032

Catalog No. PIPA5142032

Description

This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000. Preliminary analysis showed no signal in Western Blot.

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). [UniProt].

Specifications

• Antigen: UBE2O
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE2O
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme; (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O; 9630022H21; B230113M03Rik; E2/E3 hybrid ubiquitin-protein ligase UBE2O; E2-230K; Kiaa1734; mKIAA1734; RGD1310297; UBE2O; Ubiquitin carrier protein O; ubiquitin conjugating enzyme E2 O; ubiquitin conjugating enzyme E2O; ubiquitinating enzyme E2-230 kDa; ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2O; ubiquitin-protein ligase O
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 63893
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin)
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q9C0C9
• Gene Symbols: UBE2O
• Immunogen: Peptide with sequence ALLEAGMPECTEDK.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG