Invitrogen™ UBE2O Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA531429

Catalog No. PIPA531429

Description

Recommended positive controls: 293T, HeLa. Predicted reactivity: Mouse (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950). Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138). Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153). Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1. Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950). Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate 'Lys-63'-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). [UniProt].

Specifications

• Antigen: UBE2O
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE2O
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme; (E3-independent) E2 ubiquitin-conjugating enzyme UBE2O; 9630022H21; B230113M03Rik; E2/E3 hybrid ubiquitin-protein ligase UBE2O; E2-230K; Kiaa1734; mKIAA1734; RGD1310297; UBE2O; Ubiquitin carrier protein O; ubiquitin conjugating enzyme E2 O; ubiquitin conjugating enzyme E2O; ubiquitinating enzyme E2-230 kDa; ubiquitin-conjugating enzyme E2 O; Ubiquitin-conjugating enzyme E2 of 230 kDa; ubiquitin-conjugating enzyme E2-230K; ubiquitin-conjugating enzyme E2O; ubiquitin-protein ligase O
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 217342, 63893
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q6ZPJ3, Q9C0C9
• Gene Symbols: UBE2O
• Immunogen: Recombinant protein encompassing a sequence within the center region of human UBE2O. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG