Invitrogen™ UBE2U Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA576373

Catalog No. PIPA576373

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by a Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2U (ubiquitin-conjugating enzyme E2U) is a 321 amino acid E2 ubiquitin conjugating enzyme that catalyzes the covalent attachment of ubiquitin to other proteins. Existing as two alternatively spliced isoforms, UBE2U is encoded by a gene located on human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome.

Specifications

• Antigen: UBE2U
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE2U
• Gene Alias: E2 ubiquitin-conjugating enzyme U; MGC35130; RP4-636O23.1; testicular tissue protein Li 216; UBE2U; Ubiquitin carrier protein U; ubiquitin conjugating enzyme E2 U (putative); ubiquitin conjugating enzyme E2U (putative); ubiquitin-conjugating enzyme E2 U; ubiquitin-conjugating enzyme E2U (putative); ubiquitin-protein ligase U
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 148581, 381534
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q5VVX9
• Gene Symbols: UBE2U
• Immunogen: Recombinant fusion protein of human UBE2U.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG