Invitrogen™ UBE2V2 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA141467

Catalog No. 01-675-295

Description

Suggested positive control: HL-60, antigen standard for UBE2V2 (transient overexpression lysate).

The differentiation and activation of monocytes (MO) and monocytic cells is modulated by 1alpha,25-dihydroxyvitamin D3 (Vitamin D3). Recently, using mRNA Differential Display technology, Fritsche J, et al have identified a gene which is induced in freshly isolated human blood MO that are cultured for 4 hours with Vitamin D3. This cDNA encodes a putative 16.5 kD protein. The product of this gene has structural homology with yeast MMS2p and human CROC-1), which defines a structurally and functionally conserved Ubc-like protein family. This protein was called Ubiquitin E2 variant (UBE2V2) or MMS2 (methyl methanesulfonate sensitive 2). The yeast UEV protein, Mms2, is required together with Ubc13 for RAD6/RAD18-dependent postreplicative DNA repair in yeast. These proteins also play a central role in the assembly of K63-linked polyubiquitin chains (4,5) UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and IkBa kinase activation. Recent crystal structure analysis provides direct evidence that the Mms2/Ubc13 heterodimer is necessary for DNA repair.

Specifications

• Antigen: UBE2V2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBE2V2
• Gene Alias: 1 alpha,25-dihydroxyvitamin D3-inducible; 1110021H13Rik; 4632410D19Rik; 5730524P06Rik; 6820402M05; AI848315; C81524; DDVit 1; DDVIT1; DDVit-1; EDAF-1; EDPF1; EDPF-1; enterocyte differentiation promoting factor; enterocyte differentiation-associated factor 1; enterocyte differentiation-associated factor EDAF-1; enterocyte differentiation-promoting factor 1; meth; methyl methanesulfonate sensitive 2; methyl methanesulfonate sensitive 2, S. cerevisiae, homolog of; Mms2; MMS2 homolog; ubc-like protein MMS2; UBE2V2; ubiquitin conjugating enzyme E2 V2; ubiquitin conjugating enzyme E2 variant 2; ubiquitin-conjugating enzyme E2 variant 2; ubiquitin-conjugating enzyme E2v2; ubiquitin-conjugating enzyme variant MMS2; UEV2; UEV-2; vitamin D3-inducible protein
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 7336
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.2% gelatin and 0.05% sodium azide
• Gene Accession No.: Q15819
• Gene Symbols: UBE2V2
• Immunogen: Mixture of synthetic peptides containing amino acids 12-28 of human UBE2V2.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG