Ubiquitin Activating Enzyme E1Rabbit anti-Human, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200401888

Catalog No. 50-199-0846

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is directed against human Ubiquitin Activating Enzyme E1 protein. A BLAST analysis was used to suggest that this antibody would react with Ubiquitin Activating Enzyme E1 protein from human (100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.

UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11. 23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.

Specifications

• Antigen: Ubiquitin Activating Enzyme E1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: UBA1
• Gene Alias: A1S9; A1S9 protein; A1S9T; A1S9T and BN75 temperature sensitivity complementing; A1ST; AMCX1; CFAP124; EMBL:AAH85791.1, ECO:0000312; GXP1; POC20; POC20 centriolar protein homolog; Protein A1S9; RGD:1359327}; Sbx; SMAX2; testicular secretory protein Li 63; Uba1; uba1 {ECO:0000312; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; Ube-1; Ube1ax; Ube1x; ubiquitin like modifier activating enzyme 1; Ubiquitin-activating enzyme E1; ubiquitin-activating enzyme E1 {ECO:0000250; Ubiquitin-activating enzyme E1 X; ubiquitin-activating enzyme E1, Chr X; ubiquitin-like modifier activating enzyme 1; Ubiquitin-like modifier-activating enzyme 1; ubiquitin-like modifier-activating enzyme 1 {ECO:0000250; Ubiquitin-like modifier-activating enzyme 1 X; UniProtKB:Q02053}
• Host Species: Rabbit
• Quantity: 500 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1.2 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P22314
• Gene Symbols: UBA1
• Immunogen: Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
• Regulatory Status: RUO
• Gene ID (Entrez): 7317
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized