Invitrogen™ Ubiquitin Monoclonal Antibody (FK1), FITC

Catalog No.	Quantity
PIMA545326	100 μg

Catalog No. PIMA545326

Supplier: Invitrogen™ MA545326

Description

Mouse Monoclonal Antibody

A 1:5000 dilution of MA5-45326 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

Specifications

• Antigen: Ubiquitin
• Applications: ELISA, Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: FK1
• Concentration: 1 mg/mL
• Conjugate: FITC
• Formulation: PBS, 9.09mM sodium bicarbonate with 640.91mM DMSO, 136.36mM ethanolamine and no preservative; pH 7.4
• Gene: UBB
• Gene Accession No.: P0CG47, P0CG48, P62979, P62987, Q71UM5
• Gene Alias: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; 40S ribosomal protein S27a; 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; AI194771; AL033289; CEP80; CG5271; CG5271-PA; Chap1; Dmel\CG5271; Dmel_CG5271; Dub80; epididymis luminal protein 112; epididymis secretory protein Li 50; HEL112; HEL-S-50; hm:zeh0386; HMG20; hPLIC-2; HRIHFB2157; HUBCEP52; ik:tdsubc_1f2; l(2)04820; l(2)31Ei; LOC100912032; LOC101747587; male female sterile 31; mfs(2)31; mfs(2)48; mfs48; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; poly-ubiquitin precursor; polyubiquitin-B; Polyubiquitin-C; polyubiquitin-C-like; RE44350p; ribosomal protein S27; ribosomal protein S27 like; ribosomal protein S27A; ribosomal protein S27a L homeolog; ribosomal protein S27a S homeolog; ribosomal protein S27-like; ribosomal protein S27-like protein; RPL40; RpS27; Rps27a; rps27a.L; rps27a.S; RpS27A-PA; RPS27L; S27A; similar to polyubiquitin; similar to ubiquitin-S27a fusion protein; Small ribosomal subunit protein eS27-like; Small ribosomal subunit protein eS31; tdsubc_1f2; TI-225; UB3-D; Uba52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP80; Ubi-f80; Ubi-m; Ubiq; Ubiquilin 2; ubiquitin; ubiquitin (34 AA) (1 is 2nd base in codon); ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; ubiquitin carboxyl extension protein 80; ubiquitin copy 4; ubiquitin fusion 80; ubiquitin polyprotein (heat shock related); ubiquitin/ribosomal protein; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80; ubiquitin-hybrid protein precursor; Ubiquitin-monomeric; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2; ubiquitin-S27a fusion protein; Unknown (protein for MGC:127652); unnamed protein product; XELAEV_18028796mg; xx:tdsubc_1f2; zgc:66168; zgc:66168 protein
• Gene Symbols: RPS27A, RPS27L, UBA52, UBB, UBC
• Host Species: Mouse
• Immunogen: Ubiquitin conjugated lysozyme.
• Purification Method: Size-exclusion - Gel filtration
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 51065, 6233, 7311, 7314, 7316
• Target Species: Human, All species
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgM
• Target Molecule: Chemical

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov