Ubiquitin Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200401431S

Catalog No. 50-199-0831

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

Specifications

• Antigen: Ubiquitin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RPS27A
• Gene Alias: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; 40S ribosomal protein S27a; 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; AI194771; AL033289; BOS_10808; BOS_11463; BOS_16579; BOS_18906; CEP80; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; HEL112; HEL-S-50; HMG20; hPLIC-2; HRIHFB2157; HUBCEP52; LOC100912032; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; ribosomal protein S27; ribosomal protein S27 like; ribosomal protein S27a; ribosomal protein S27a L homeolog; ribosomal protein S27a S homeolog; ribosomal protein S27-like; ribosomal protein S27-like protein; RPL40; RPS27A; rps27a.L; rps27a.S; Rps27l; S27A; Small ribosomal subunit protein eS27-like; Small ribosomal subunit protein eS31; TI-225; Uba52; UBA80; Ubb; Ubb2; Ubc; UBCEP1; UBCEP80; Ubiquilin 2; Ubiquitin; ubiquitin and ribosomal protein S27a; ubiquitin B; ubiquitin C; Ubiquitin carboxyl extension protein 80; ubiquitin/ribosomal protein; Ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-CEP80; Ubiquitin-related; Ubiquitin-related 1; Ubiquitin-related 2; XELAEV_18028796mg
• Host Species: Rabbit
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Multi-species, Mouse, Rat
• Product Type: Antibody

• Applications: ELISA, Immunohistochemistry, Western Blot
• Concentration: 5 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P0CG47, P0CG48, P0CG49, P0CG50, P0CG51, P24051, P62979, P62982, P62983, Q63429, Q6ZWY3, Q71UM5
• Gene Symbols: RPS27A, RPS27L, UBB, UBC
• Immunogen: This purified antibody was prepared from rabbit serum after repeated immunizations with ubiquitin coupled to rabbit IgG using glutaraldehyde.
• Regulatory Status: RUO
• Gene ID (Entrez): 100912032, 192255, 22187, 22190, 50522, 51065, 6233, 67941, 681429, 7314, 7316, 78294
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized