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Description
Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells.
- Uncoupling protein 2 (mitochondrial, proton carrier)
- Transfected Cell Line: 293T
- Plasmid: pCMV-UCP2 full-length
- Host: Human
- Theoretical MW: 33.73kDa
- Storage Buffer: 1X Sample Buffer (50mM Tris-HCl, 2% SDS, 10% glycerol, 300mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Western Blotting
Specifications
Specifications
| Cell Line | 293T |
| For Use With (Application) | Western Blot |
| Description | UCP2 293T Cell Transient Overexpression Lysate(Denatured) |
| Gene Accession No. | BC011737 |
| Molecular Weight (g/mol) | 33.73 |
| pH Range | 6.8 |
| Plasmid | pCMV-UCP2 full-length |
| Quality Control Testing | Transient overexpression cell Lysate was tested with Anti-UCP2 antibody (H00007351-B01) by western blotting |
| Quantity | 100 μL |
| Storage Buffer | 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue) |
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