Invitrogen™ UFD1L Monoclonal Antibody (2A6F3)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA538653

Catalog No. PIMA538653

Description

This antibody has been tested in indirect ELISA.

Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then potentiates the proteolytic degradation of these protein conjugates by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or alternatively by the Ub-fusion degradation (UFD) pathway. The UBP pathway targets the C-terminal glycine residue on Ub that is involved in the formation of Ub-conjugates, while UFD proteins preferentially cleave Ub-conjugated proteins that contain an amino acid substitution at this glycine residue. The UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate. Mutations within the UFD1 gene are implicated in the development of CATCH22 syndrome, which is characterized by cardiac defects, cleft palate and hypocalcemia, suggesting that this proteolytic pathway may be involved in the progression of these developmental defects.

Specifications

• Antigen: UFD1L
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.05% sodium azide
• Gene Accession No.: Q92890
• Gene Symbols: UFD1
• Immunogen: Purified recombinant fragment of human UFD1L (aa: 208-307) expressed in E. Coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 2A6F3
• Conjugate: Unconjugated
• Gene: UFD1
• Gene Alias: RGD:619822}; UB fusion protein 1; UB fusion protein 1 {ECO:0000250; ubiquitin fusion degradation 1 like; ubiquitin fusion degradation 1 like (yeast); Ubiquitin fusion degradation protein 1; Ubiquitin fusion degradation protein 1 homolog; ubiquitin fusion degradation protein 1 homolog {ECO:0000250; Ubiquitin recognition factor in ER-associated degradation protein 1; UFD1; UFD1L; ufd1l {ECO:0000312; UniProtKB:Q92890}
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 7353
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid