ULK1 Recombinant Rabbit Monoclonal Antibody (JA58-36), Invitrogen™

Rabbit Recombinant Monoclonal Antibody

Supplier:  Thermo Scientific MA532699

Catalog No. PIMA532699

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.

Specifications

• Antigen: ULK1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 0.05% BSA, 40% Glycerol and 0.05% sodium azide, pH 7.4
• Gene Accession No.: O70405, O75385
• Gene Symbols: ULK1
• Immunogen: Synthetic peptide within Human ULK1 aa 930-979
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Clone: JA58-36
• Conjugate: Unconjugated
• Gene: ULK1
• Gene Alias: APG1, ATG 1, ATG1, ATG1 autophagy related 1 homolog, ATG1A, AU041434, Autophagy-related protein 1 homolog, C. elegans, EC:2.7.11.1, FLJ38455, hATG1, KIAA0722, mKIAA0722, Serine/threonine-protein kinase ULK1, serine/threonine-protein kinase Unc51.1, ULK 1, Ulk1, UNC 51, UNC51, unc-51 like autophagy activating kinase 1, unc-51 like kinase 1, Unc51.1, unc-51-like kinase 1
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 22241, 360827, 8408
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid