Invitrogen™ uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA3030

Catalog No. MA3030

Description

MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues. MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present. The MA3-030 antigen is purified bovine bNOS.

NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

Specifications

• Antigen: uNOS
• Classification: Monoclonal
• Concentration: Conc. Not Determined
• Formulation: Ascites with 0.05% sodium azide
• Gene Accession No.: P29473, P29474, P29475, P29476, P29477, P35228, P70313, Q06518, Q27995, Q62600, Q9Z0J4
• Gene Symbols: NOS1, NOS2, Nos3
• Immunogen: Purified bovine bNOS.
• Regulatory Status: RUO
• Gene ID (Entrez): 18125, 18126, 18127, 24598, 24599, 24600, 282876, 287024, 4842, 4843, 4846, 536132
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: NOS-3F7-B11 B5
• Conjugate: Unconjugated
• Gene: NOS2
• Gene Alias: 2310005C01Rik; 2310065A03Rik; bNOS; cNOS; constitutive NOS; Ecnos; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial NOS; eNOS; Hepatocyte NOS; HEP-NOS; IHPS1; inducible nitric oxid synthase; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; INOS; i-NOS; Inosl; MAC-NOS; macrophage inducible nitric oxide synthase; macrophage NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; nitric oxide synthase 3, endothelial cell; nitric oxide synthase II; nitric oxide synthase, brain; Nitric oxide synthase, endothelial; nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric-oxide synthase; nNOS; N-NOS; NO; NOS; NOS type I; NOS type II; NOS type III; NOS, type II; Nos1; Nos-1; NOS2; Nos-2; Nos2a; Nos3; Nos-3; NOS-I; NOSII; NOS-II; NOSIII; Peptidyl-cysteine S-nitrosylase NOS1; peptidyl-cysteine S-nitrosylase NOS2
• Host Species: Mouse
• Quantity: 200 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Bovine
• Product Type: Antibody
• Isotype: IgM

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov