uPAR (CD87), Mouse anti-Hamster, Clone: 5D6, Millipore Sigma™

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma MABC1099100UG

Catalog No. MABC1099100

Description

Anti-uPAR (CD87), clone 5D6, Cat. No. MABC1099, is a mouse monoclonal antibody that detects Urokinase plasminogen activator surface receptor and has been tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

Urokinase plasminogen activator surface receptor (UniProt: Q9R119; also known as uPAR) is encoded by the Upar gene (Gene ID: 100689422) in Chinese hamster. uPAR is a GPI-anchored cell membrane receptor for urokinase plasminogen activator (uPA), an enzyme responsible for the activation of plasmin and degradation of extracellular matrix components. Higher expression of uPAR has been reported in many human cancers, including non-small cell lung cancer and colorectal cancer. Its higher levels correlate with poor prognosis and early invasion and metastasis. uPAR is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form, which contains three homologus domains (DI, DII, and DIII). uPAR is also reported to serve as an adhesion receptor for vitronectin and a direct interaction between uPAR and vitronectin is shown to be important for inducing changes in cell morphology, cell migration, and signaling. Blocking of uPAR activity can impair cell adhesion and migration in RAS mutated cancer cells. (Ref.: Di Mauro, C et al. (2017). Scientific Reports 7, Article number: 9388).

Specifications

• Antigen: uPAR (CD87)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene Symbols: Upar
• Immunogen: Raft fraction of the plasma membrane from Chinese hamster ovary (CHO) cells.
• Quantity: 100 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): XP_007647182.1
• Target Species: Hamster
• Form: Purified

• Applications: Flow Cytometry, Immunocytochemistry, Western Blot
• Clone: 5D6
• Dilution: Western Blotting Analysis: A representative lot detected uPAR (CD87) in Western Blotting applications (Maeda, Y., et. al. (2007). Mol Biol Cell. 18(4):1497-506; Lee, G.H., et. al. (2016). J Cell Biol. 215(5):705-718; Hirata, T., et. al. (2018). Nat Commun. 9(1):405).Flow Cytometry Analysis: A representative lot detected uPAR (CD87) in Flow Cytometry applications (Ashida, H., et. al. (2006). J Biol Chem. 281(2):896-904; Lee, G.H., et. al. (2016). J Cell Biol. 215(5):705-718; Hirata, T., et. al. (2018). Nat Commun. 9(1):405; Hirata, T., et. al. (2013). J Biochem. 154(3):257-64).Immunocytochemistry Analysis: A representative lot detected uPAR (CD87) in Immunocytochemistry applications (Kanzawa, N., et. al. (2012). J Lipid Res. 53(4):653-63).
• Gene Alias: Urokinase plasminogen activator surface receptor;CD87
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 5D6 detects urokinase plasminogen activator surface receptor. It targets an epitope with in the extracellular domain.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Isotype: IgG1 κ