Invitrogen™ USP13 Monoclonal Antibody (OTI4G11)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA524904

Catalog No. PIMA524904

Description

USP13 Monoclonal Antibody for Western Blot, IHC (P), IP

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt].

Specifications

• Antigen: USP13
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q92995
• Gene Symbols: USP13
• Immunogen: Full-length protein expressed in 293T cell transfected with human USP13 expression vector.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Canine, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Clone: OTI4G11
• Conjugate: Unconjugated
• Gene: USP13
• Gene Alias: 2700071E21Rik; AI848077; Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT3; IsoT-3; Ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin thioesterase 13; ubiquitin thiolesterase 13; ubiquitin-specific-processing protease 13; usp13; wu:fc61g08
• Host Species: Mouse
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 478640, 8975
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid