Invitrogen™ USP13 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5109268

Catalog No. PIPA5109268

Description

Positive Control: 293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63.

Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt].

Specifications

• Antigen: USP13
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: USP13
• Gene Alias: 2700071E21Rik; AI848077; Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT3; IsoT-3; Ubiquitin carboxyl-terminal hydrolase 13; ubiquitin specific peptidase 13; ubiquitin specific peptidase 13 (isopeptidase T-3); ubiquitin specific protease 13 (isopeptidase T-3); Ubiquitin thioesterase 13; ubiquitin thiolesterase 13; ubiquitin-specific-processing protease 13; usp13; wu:fc61g08
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8975
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles, store in dark
• Form: Liquid

• Applications: Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 0.2% BSA, 50% glycerol and 0.05% sodium azide; pH 7.4
• Gene Accession No.: Q92995
• Gene Symbols: USP13
• Immunogen: Recombinant protein within Human USP13 aa 570-776.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG