USP7 Polyclonal Antibody, Bethyl Laboratories

Catalog No.
50-155-6771	20 μL; Unconjugated

Catalog No. 50-155-6771 Supplier Bethyl Laboratories, Inc Supplier No. A300033AT

Description

Rabbit Polyclonal Antibody

The recommended shelf life for this product is 1 year from date of receipt. Application Note: For IHC, many tissues will not require epitope retrieval. However to boost weak staining epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Based on 100% sequence identity, this antibody is predicted to react with Rat

USP7 or HAUSP is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, HAUSP activity generally stabilizes its substrate proteins. HAUSP is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53. Normally, p53 levels are kept low due to Mdm2-mediated ubiquitylation and degradation of p53. Interestingly, in response to oncogenic insults, HAUSP can deubiquitinate p53 and protect p53 from Mdm2-mediated degradation, indicating that it may possess a tumor suppressor function for the immediate stabilization of p53 in response to stress. Another important role of HAUSP function involves the oncogenic stabilization of p53. Oncogenes such as Myc and E1A are thought to activate p53 through a p19 alternative reading frame (p19ARF, also called ARF)-dependent pathway, although some evidence suggests ARF is not essential in this process. An intriguing possibility is that HAUSP provides an alternative pathway for safeguarding the cell against oncogenic insults.

Specifications

• Antigen: USP7
• Applications: Immunohistochemistry, Western Blot
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: TBS with 0.1% BSA and 0.09% sodium azide
• Gene: USP7
• Gene Accession No.: Q6A4J8, Q93009
• Gene Alias: HAUSP
• Gene Symbols: Usp7
• Host Species: Rabbit
• Immunogen: Between 1 and 50
• Purification Method: Antigen affinity chromatography
• Quantity: 20 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 252870, 7874
• Target Species: Human, Mouse
• Content And Storage: 4° C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG