Vβ 10[b] TCR Rat anti-Mouse, Brilliant Violet 650, Clone: B21.5, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 742822

Catalog No. BDB742822

Description

The B21.5 monoclonal antibody specifically recognizes the Vβ10 T-Cell Receptor (TCR) of mice having the b /haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb /gene complex. It does not react with mice having the a /(eg, C57BR, C57L, SJL, SWR) or c /(eg, RIII) Tcrb /haplotype.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.

Specifications

• Antigen: Vβ 10[b] TCR
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR Vβ10, TCR V beta 10, TCR Vb10
• Immunogen: Mouse Cytotoxic T-Cell Clone Soluble αβ TCR
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV650 under optimal conditions that minimize unconjugated dye and antibody.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: B21.5
• Conjugate: Brilliant Violet 650
• Format: Affinity Purified
• Host Species: Rat
• Isotype: Rat LOU, also known as Louvain, LOU/C, LOU/M IgG_2a, λ
• Quantity: 50μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal