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Vβ 10[b] TCR Rat anti-Mouse, Brilliant Violet 650, Clone: B21.5, BD Optibuild™

Rat Monoclonal Antibody

Manufacturer:  BD Biosciences 742822

Catalog No. BDB742822


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Description

Description

The B21.5 monoclonal antibody specifically recognizes the Vβ10 T-Cell Receptor (TCR) of mice having the b /haplotype (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb /gene complex. It does not react with mice having the a /(eg, C57BR, C57L, SJL, SWR) or c /(eg, RIII) Tcrb /haplotype.

The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm. Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors. BV650 will have moderate spillover into the BD Horizon™ BV711 detector.
Specifications

Specifications

Vβ 10[b] TCR
B21.5
Brilliant Violet 650
TCR Vβ10, TCR V beta 10, TCR Vb10
Mouse Cytotoxic T-Cell Clone Soluble αβ TCR
50 μg
Primary
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
Rat
Affinity Purified
RUO
Mouse
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a λ
SDS
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